Ultra-Fast Brillouin Light-Sheet Microscopy for Real-Time Mechanical Imaging in Biology

Objective

Understanding the mechanical properties of biological systems is crucial for unraveling key processes in development, disease progression, and cellular function. This ERC Proof-of-Concept project proposes the development of a next-generation Brillouin microscope, inspired by light-sheet microscopy principles, to enable ultra-fast, high-resolution, and label-free imaging of mechanical properties in live biological samples. Traditional Brillouin microscopy has been limited by slow acquisition speeds and photodamage, hindering its practical application in dynamic biological contexts. Our novel system integrates planar illumination and highly multiplexed spectral detection to dramatically increase imaging speed while minimizing phototoxicity and preserving mechanical contrast. This platform will empower researchers to study biomechanical dynamics in real-time, from embryonic development to cancer progression, with unprecedented temporal and spatial resolution. The project will also explore commercialization pathways, with potential applications in biomedical research, drug screening, and diagnostic tools. By demonstrating technical feasibility, validating biological relevance, and assessing market potential, this project bridges the gap between fundamental photonics research and impactful biomedical innovation.